代表性论文
| • Han, D., Xiao, Q., Wang, Y., Zhang, H., Dong, X., Li, G., Kong, X., Wang, S., Song, J., Zhang, W., Zhou, J., Bi, L., Yuan, Y., Shi, L., Zhong, N., Yang, H.*, Zhou, Y.* (2023) Development of miniature base editors using engineered IscB nickase. Nat. Methods. : In Press. |
| • Tong, H., Huang, J.*, Xiao,Q., He, B., Dong, X., Liu, Y., Yang, X., Han, D., Wang, Z., Ying, W., Zhang, R., Wei, Y., Wang, X., Xu, C., Zhou, Y., Li, Y., Cai, M., Wang, Q., Xue, M., Li, G., Fang, K., Zhang, H.*, Yang, H.* (2022) High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effect. Nat. Biotechnol. : In Press. |
| • Xiao, Q., Xu, Z., Xue, Y., Xu, C., Han, L., Liu, Y., Wang, F., Zhang, R., Han, S., Wang, X., Li, G., Li, H*., Yang, H.*, Shu, Y.* (2022) Rescue of autosomal dominant hearing loss by in vivo delivery of mini dCas13X-derived RNA base editor. Sci. Transl. Med. 14: 654. |
| • Xu, C., Zhou, Y.*, Xiao, Q., He, B., Geng, G., Wang, Z., Cao, B., Dong, X., Bai, W., Wang, Y., Wang, X., Zhou, D., Yuan, T., Huo, X., Lai, J.*, Yang, H.* (2021) Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes. Nat. Methods 18: 499-506. |
| • Gao, N., Hu, J., He, B., Ji, Z., Hu, X., Huang, J., Wei, Y., Peng, J., Wei, Y., Zhou, Y., Shen, X., Li, H., Feng, X., Xiao, Q., Shi, L., Sun, Y., Zhou, C., Zhou, H.* & Yang, H.* (2021) Endogenous promoter-driven sgRNA for monitoring the expression of low-abundance transcripts and lncRNAs. Nat. Cell Biol. 23: 99-108. |
| • Zuo, E., Sun, Y., Wei, W., Yuan, T., Ying, W., Sun, H., Yuan, L., Steinmetz, L.*, Li, Y.* & Yang H.* (2020) GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos. Nat. Protoc. 15: 3009-3029. |
| • Zuo, E.*, Sun, Y., Yuan, T., He, B., Zhou, C., Ying, W., Liu, J., Wei, W., Zeng, R., Li, Y.* & Yang H.* (2020) A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects. Nat. Methods 17: 600-604. |
| • Zhou, H.*, Su, J., Hu, X., Zhou, C., Li, H., Chen, Z., Xiao, Q., Wang, B., Wu, W., Sun, Y., Zhou, Y., Tang, C., Liu, F., Wang, L., Feng, C., Liu, M., Li, S., Zhang, Y., Xu, H., Yao, H., Shi, L. & Yang H.* (2020) Glia-to-neuron conversion by CRISPR-CasRx alleviates symptoms of neurological disease in mice. Cell 181: 590-603. |
| • Zuo, E., Sun, Y., Wei, W., Yuan, T., Ying, W., Sun, H., Yuan, L., Steinmetz, L. M.*, Li, Y.* & Yang, H.* (2019) Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos. Science 364: 289-292. |
| • Zhou, C., Sun, Y., Yan, R., Liu, Y., Zuo, E., Gu, C., Han, L., Wei, Y., Hu, X., Zeng, R., Li, Y.*, Zhou, H.*, Guo, F.* & Yang H.* (2019) Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature 517: 275-278. |
| • Zhou, H., Liu, J., Zhou, C., Gao, N., Rao, Z., Li, H., Hu, X., Li, C., Yao, X., Shen, X., Sun, Y., Wei, Y., Liu, F., Ying, W., Zhang, J., Tang, C., Zhang, X., Xu, H., Shi, L., Cheng, L., Huang, P.* & Yang, H.* (2018) In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice. Nat. Neurosci. 21: 440-446. |
| • Yao, X.*, Zhang, M., Wang, X., Ying, W., Hu, X., Dai, P., Meng, F., Shi, L., Sun, Y., Yao, N., Zhong, W., Li, Y., Wu, K., Li, W.*, Chen, Z.* & Yang, H.* (2018) Tild-CRISPR allows for efficient and precise gene knockin in mouse and human cells. Dev. Cell 45: 526-536. |
| • Zuo, E., Huo, X., Yao, X., Hu, X., Sun, Y., Yin, J., He, B., Wang, X., Shi, L., Ping, J., Wei, Y., Ying, W., Wei, W., Liu, W., Tang, C., Li, Y., Hu, J.* & Yang, H.* (2017) CRISPR/Cas9-mediated targeted chromosome elimination. Genome Biol. 18: 224. |
所有论文
| • Han, D., Xiao, Q., Wang, Y., Zhang, H., Dong, X., Li, G., Kong, X., Wang, S., Song, J., Zhang, W., Zhou, J., Bi, L., Yuan, Y., Shi, L., Zhong, N., Yang, H.*, Zhou, Y.* (2023) Development of miniature base editors using engineered IscB nickase. Nat. Methods. : In Press. |
| • Tong, H., Huang, J.*, Xiao,Q., He, B., Dong, X., Liu, Y., Yang, X., Han, D., Wang, Z., Ying, W., Zhang, R., Wei, Y., Wang, X., Xu, C., Zhou, Y., Li, Y., Cai, M., Wang, Q., Xue, M., Li, G., Fang, K., Zhang, H.*, Yang, H.* (2022) High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effect. Nat. Biotechnol. : In Press. |
| • Xiao, Q., Xu, Z., Xue, Y., Xu, C., Han, L., Liu, Y., Wang, F., Zhang, R., Han, S., Wang, X., Li, G., Li, H*., Yang, H.*, Shu, Y.* (2022) Rescue of autosomal dominant hearing loss by in vivo delivery of mini dCas13X-derived RNA base editor. Sci. Transl. Med. 14: 654. |
| • Xu, C., Zhou, Y.*, Xiao, Q., He, B., Geng, G., Wang, Z., Cao, B., Dong, X., Bai, W., Wang, Y., Wang, X., Zhou, D., Yuan, T., Huo, X., Lai, J.*, Yang, H.* (2021) Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes. Nat. Methods 18: 499-506. |
| • Gao, N., Hu, J., He, B., Ji, Z., Hu, X., Huang, J., Wei, Y., Peng, J., Wei, Y., Zhou, Y., Shen, X., Li, H., Feng, X., Xiao, Q., Shi, L., Sun, Y., Zhou, C., Zhou, H.* & Yang, H.* (2021) Endogenous promoter-driven sgRNA for monitoring the expression of low-abundance transcripts and lncRNAs. Nat. Cell Biol. 23: 99-108. |
| • Ling, S., Yang, S., Hu, X., Yin, D., Dai, Y., Qian, X., Wang, D., Pan, X., Hong, J., Sun, X., Yang, H., Paludan, S. & Cai Y. * (2021) Lentiviral delivery of co-packaged？Cas9？mRNA and a？Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice. Nat. Biomed. Eng. 5: 144-156. |
| • Fang, K., Li, Q., Wei, Y., Zhou, C., Guo, W., Shen, J., Wu, R., Ying, W., Yu, L., Zi, J., Zhang, Y., Yang, H.*, Liu, S.* & Chen, D.* (2021) Prediction and validation of mouse meiosis-essential genes based on spermatogenesis proteome dynamics. Mol. Cell Proteomics. 20: 100014. |
| • Liu, Y., Zhou, C., Huang, S., Dang, L., Wei, Y., He, J., Zhou, Y., Mao, S., Tao, W., Zhang, Y., Yang, H.*, Huang, X.* & Chi, T.* (2020) A Cas-embedding strategy for minimizing off-target effects of DNA base editors. Nat. Commun. 11: 6073. |
| • Zuo, E., Sun, Y., Wei, W., Yuan, T., Ying, W., Sun, H., Yuan, L., Steinmetz, L.*, Li, Y.* & Yang H.* (2020) GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos. Nat. Protoc. 15: 3009-3029. |
| • Zuo, E.*, Sun, Y., Yuan, T., He, B., Zhou, C., Ying, W., Liu, J., Wei, W., Zeng, R., Li, Y.* & Yang H.* (2020) A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects. Nat. Methods 17: 600-604. |
| • Lin, X., Chen, H., Lu, Y., Hong, S., Hu, X., Gao, Y., Lai, L., Li, J., Wang, Z., Ying, W., Ma, L., Wang, N., Zuo, E.*, Yang, H.* & Chen, W.* (2020) Base editing-mediated splicing correction therapy for spinal muscular atrophy. Cell Res. 30: 548-550. |
| • He, B., Peng, W., Huang, J., Zhang, H., Zhou, Y., Yang, X., Liu, J., Li, Z., Xu, C., Xue, M., Yang H.* & Huang, P.* (2020) Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver. Protein Cell 11: 518-524. |
| • Zhou, C., Hu, X., Tang, C., Liu, W., Wang, S., Zhou, Y., Zhao, Q., Bo, Q., Shi, L., Sun, X.*, Zhou, H.* & Yang H.* (2020) CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration. Natl. Sci. Rev. 7: 835-837. |
| • Zhou, H.*, Su, J., Hu, X., Zhou, C., Li, H., Chen, Z., Xiao, Q., Wang, B., Wu, W., Sun, Y., Zhou, Y., Tang, C., Liu, F., Wang, L., Feng, C., Liu, M., Li, S., Zhang, Y., Xu, H., Yao, H., Shi, L. & Yang H.* (2020) Glia-to-neuron conversion by CRISPR-CasRx alleviates symptoms of neurological disease in mice. Cell 181: 590-603. |
| • Li, J., Lin, X., Tang, C., Lu, Y., Hu, X., Zuo, E., Li, H., Ying, W., Sun, Y., Lai, L., Chen, H., Guo, X., Zhang, Q., Wu, S., Zhou, C., Shen, X., Wang, Q., Lin, M., Ma, L., Wang, N., Krainer A.R., Shi, L.*, Yang, H.* & Chen W.* (2020) Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice. Natl. Sci. Rev. 7: 92-101. |
| • Yang, G., Zhou, C., Wang, R., Huang, S., Wei, Y., Yang, X., Liu, Y., Li, J., Lu, Z., Ying, W., Li, X., Jing, N., Huang, X.*, Yang, H.* & Qiao, Y.* (2019) Base-editing-mediated R17H substitution in histone H3 reveals methylation-dependent regulation of Yap signaling and early mouse embryo development. Cell Rep. 26: 302-312. |
| • Zuo, E., Sun, Y., Wei, W., Yuan, T., Ying, W., Sun, H., Yuan, L., Steinmetz, L. M.*, Li, Y.* & Yang, H.* (2019) Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos. Science 364: 289-292. |
| • Wang, H.* & Yang, H.* (2019) Gene-edited babies: What went wrong and what could go wrong. PLOS Biol. 17: e3000224. |
| • Zhang, M., Zhou, C., Wei, Y., Xu, C., Pan, H., Ying, W., Sun, Y., Sun, Y., Xiao, Q., Yao, N., Zhong, W., Li, Y., Wu, K., Yuan, G., Mitalipov, S.*, Chen, Z.* & Yang, H.* (2019) Human cleaving embryos enable robust homozygotic nucleotide substitutions by base editors. Genome Biol. 20:101.: |
| • Zhou, C., Sun, Y., Yan, R., Liu, Y., Zuo, E., Gu, C., Han, L., Wei, Y., Hu, X., Zeng, R., Li, Y.*, Zhou, H.*, Guo, F.* & Yang H.* (2019) Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature 517: 275-278. |
| • Liu, Y., Li, J., Zhou, C., Meng, B., Wei, Y., Yang, G., Lu, Z., Shen, Q., Zhang, Y., Yang, H.* & Qiao, Y.* (2019) Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9 (SaCas9). Science Bull. 64: 1592-1600. |
| • Yang, H.* (2019) Funding research, a Chinese perspective. Genome Biol. 20:177.: |
| • Hu, X., Wang, J., Yao, X., Xiao, Q., Xue, Y., Wang, S., Shi, L., Shu, Y.*, Li, H.* & Yang H.* (2019) Screened AAV variants permit efficient transduction access to supporting cells and hair cells. Cell Discov. 5: 49. |
| • Li, J., Hong, S., Chen, W.*, Zuo, E.* & Yang, H.* (2019) Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing. J. Genet. Genomics 46: 513–521. (Review) |
| • Yao, X., Liu, Z., Wang, X., Wang, Y., Nie, Y., Lai, L., Sun, R., Shi, L., Sun, Q.* & Yang, H.* (2018) Generation of knock-in cynomolgus monkey via CRISPR/Cas9 editing. Cell Res. 28: 379-382. |
| • Yao, X., Wang, X., Liu, J., Shi, L., Huang, P.* & Yang, H.* (2018) CRISPR/Cas9-mediated targeted integration in vivo using a homology-mediated end joining-based strategy. J. Vis. Exp. 133: e56844. |
| • Zhou, H., Liu, J., Zhou, C., Gao, N., Rao, Z., Li, H., Hu, X., Li, C., Yao, X., Shen, X., Sun, Y., Wei, Y., Liu, F., Ying, W., Zhang, J., Tang, C., Zhang, X., Xu, H., Shi, L., Cheng, L., Huang, P.* & Yang, H.* (2018) In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice. Nat. Neurosci. 21: 440-446. |
| • Yao, X.*, Zhang, M., Wang, X., Ying, W., Hu, X., Dai, P., Meng, F., Shi, L., Sun, Y., Yao, N., Zhong, W., Li, Y., Wu, K., Li, W.*, Chen, Z.* & Yang, H.* (2018) Tild-CRISPR allows for efficient and precise gene knockin in mouse and human cells. Dev. Cell 45: 526-536. |
| • Zhang, H., Pan, H., Zhou, C., Wei, Y., Ying, W., Li, S., Wang, G., Li, C., Ren, Y., Li, G., Ding, X., Sun, Y., Li, G., Song, L., Li, Y., Yang, H.* & Liu, Z.* (2018) Simultaneous zygotic inactivation of multiple genes in mouse through CRISPR/Cas9-mediated base editing. Development 145: dev168906. |
| • Yao, X., Wang, X., Liu, J., Hu, X., Shi, L., Shen, X., Ying, W., Sun, X., Wang, X., Huang, P.* & Yang, H.* (2017) CRISPR/Cas9–mediated precise targeted integration in vivo using a double cut donor with short homology arms. EBioMedicine 20: 19-26. |
| • Yao, X., Wang, X., Hu, X., Liu, Z., Liu, J., Zhou, H., Shen, X., Wei, Y., Huang, Z., Ying, W., Wang, Y., Nie, Y., Zhang, C., Li, S., Cheng, L., Wang, Q., Wu, Y., Huang, P., Sun, Q.*, Shi, L.* & Yang, H.* (2017) Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 27: 801-814. |
| • Zuo, E., Cai, Y., Li, K., Wei, Y., Wang, B., Sun, Y., Liu, Z., Liu, J., Hu, X., Wei, W., Huo, X., Shi, L., Tang, C., Liang, D., Wang, Y., Nie, Y., Zhang, C., Yao, X., Wang, X., Zhou, C., Ying, W., Wang, Q., Chen, R., Shen, Q., Xu, G., Li, J., Sun, Q.*, Xiong, Z.* & Yang, H.* (2017) One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs. Cell Res. 27: 933-945. |
| • Zhou, C., Zhang, M., Wei, Y., Sun, Y., Sun, Y., Pan, H., Yao, N., Zhong, W., Li, Y., Li, W.*, Yang, H.* & Chen, Z.* (2017) Highly efficient base editing in human tripronuclear zygotes. Protein Cell 8: 772-775. |
| • Zuo, E., Huo, X., Yao, X., Hu, X., Sun, Y., Yin, J., He, B., Wang, X., Shi, L., Ping, J., Wei, Y., Ying, W., Wei, W., Liu, W., Tang, C., Li, Y., Hu, J.* & Yang, H.* (2017) CRISPR/Cas9-mediated targeted chromosome elimination. Genome Biol. 18: 224. |
| • Miao, L., Yao, H., Li, C., Pu, M., Yao, X., Yang, H., Qi, X., Ren, J.* & Wang, Y.* (2016) A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1. Bba-Gene Regul. Mech. 1859: 650-662. |
| • Yang, H., Wang, H. & Jaenisch, R.* (2014) Generating genetically modified mice using CRISPR/Cas-mediated genome engineering. Nat. Protoc. 9: 1956-1968. |
| • Yang, H., Wang, H., Shivalila, C., Cheng, A., Shi, L. & Jaenisch, R.* (2013) One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell 154: 1370-1379. |
| • Cheng, A., Wang, H., Yang, H., Shi, L., Katz, Y., Theunissen, T., Rangarajan, S., Shivalila, C., Dadon, D. & Jaenisch, R.* (2013) Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 23: 1163-1171. |
| • Yang, H., Liu, Z., Ma, Y., Zhong, C., Yin, Q., Zhou, C., Shi, L., Cai, Y., Zhao, H., Wang, H., Tang, F., Wang, Y., Zhang, C., Liu, X., Lai, D., Jin, Y.*, Sun, Q.* & Li, J.* (2013) Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes. Cell Res. 23: 1187-1200. |
| • Jiang, J., Lv, W., Ye, X., Wang, L., Zhang, M., Yang, H., Okuka, M., Zhou, C., Zhang, X., Liu, L.* & Li, J.* (2013) Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation. Cell Res. 23: 92-106. |
| • Wang, H., Yang, H., Shivalila, C., Dawlaty, M., Cheng, A., Zhang, F. & Jaenisch, R.* (2013) One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153: 910-918. |
| • Shi, L., Yang, H. & Li, J.* (2012) Haploid embryonic stem cells: an ideal tool for mammalian genetic analyses. Protein Cell 3: 806-810. |
| • Yang, H., Shi, L., Wang, B., Liang, D., Zhong, C., Liu, W., Nie, Y., Liu, J., Zhao, J., Gao, X., Li, D., Xu, G.* & Li, J.* (2012) Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells. Cell 149: 605-617. |
| • Lin, J., Shi, L., Zhang, M., Yang, H., Qin, Y., Zhang, J., Gong, D., Zhang, X., Li, D. & Li, J.* (2011) Defects in trophoblast cell lineage account for the impaired in vivo development of cloned embryos generated by somatic nuclear transfer. Cell Stem Cell 8: 371-375. |
| • Yang, H., Shi, L., Chen, C. & Li, J.* (2011) Mice generated after round spermatid injection into haploid two-cell blastomeres. Cell Res. 21: 854-858. |
| • Jiang, J., Ding, G., Lin, J., Zhang, M., Shi, L., Lv, W., Yang, H., Xiao, H., Pei, G., Li, Y., Wu, J.* & Li, J.* (2011) Different developmental potential of pluripotent stem cells generated by different reprogramming strategies. J. Mol. Cell Biol. 3: 197-199. |
| • Gu, T., Guo, F., Yang, H., Wu, H., Xu, G., Liu, W., Xie, Z., Shi, L., He, X., Jin, S., Iqbal, K., Shi, Y., Deng, Z., Szabó, P., Pfeifer, G., Li, J.* & Xu, G.* (2011) The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 477: 606-610. |
| • Yang, H., Shi, L., Zhang, S., Lin, J., Jiang, J. & Li, J.* (2010) High-efficiency somatic reprogramming induced by intact MII oocytes. Cell Res. 20: 1034-1042. |
